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Small Molecule Bioanalysis

When searching for the hard-to-find molecule, gaining insights and expertise from the DMPK community is critical. Browse these resources to be even more effective in quantifying small molecules for drug efficacy.

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The Versatility of Microflow LC/MS – Making High Sensitivity Applications More Routine with ionKey/MS

Originally launched in response to the need for robust, high sensitivity microflow LC/MS applications, the integrated microfluidic tile-based ionKey/MS™ platform continues to evolve as a system that can be implemented for routine applications. ionKey/MS™ has quickly gained traction in new application spaces yielding a more functional and flexible system. These new areas include quantification of intact and digested monoclonal antibodies, thyroglobulin, opioids in oral fluid, estradiol, and steroids.

Top ten misconceptions about microflow LC/MS

You’ve heard a lot about microflow LC/MS. Are you cautious about making the switch? Do you still have questions or concerns about method development? We are going to use this time to review the top 10 misconceptions and challenges that we have heard and observed. Our hope is that after you have listened to this presentation, you will be able to make an informed decision on how microflow LC/MS will fit into your workflow.

A Sensitive Microflow LC/MS/MS Method for the Analysis of Fluticasone Propionate in Human Plasma

This poster presented at 65th ASMS Conference from June 4-8 in Indianapolis, IN.

An HS-MRM Assay for Quantification of Host Cell Proteins in Therapeutic Monoclonal Antibodies

This poster presented at 65th ASMS Conference from June 4-8 in Indianapolis, IN.

Building a Collision Cross Section Library of Pharmaceutical Drugs Using an IMS QTof Platform

This poster presented at 65th ASMS Conference from June 4-8 in Indianapolis, IN.

A Quantitative UPLC-MS/MS Research Method for the Measurement of Acetaminophen and 5 Metabolites in Plasma

This application note describes a sensitive, validated, UPLC-based bioanalytical research method for the quantification of acetaminophen and five metabolites in plasma.

The research method uses reversed-phase UPLC to obtain suitable chromatographic properties, such as retention of the analytes and the resolution of metabolites, with a run time of 7.5 min. A quantitative method was validated over the range 16 ng/mL–500 ng/mL for acetaminophen, 3.2 ng/mL–100 ng/mL for both acetaminophen glucuronide and sulfate, 0.64 ng/mL–20 ng/mL for acetaminophen cysteinyl and glutathione metabolite and 0.96 ng/mL–20 ng/mL for acetaminophen N-acetyl cysteinyl metabolite. The methodology required only 5 μL of plasma and exhibited excellent sensitivity, robustness and reproducibility.

Data Independent and Data Dependent Acquisition Strategies Combined With Ion Mobility for Drug Metabolism and Pharmacokinetics

This poster presented at 12th Annual Great Lakes DMDG meeting from May 4-5th in Michigan.

Peer reviewed literature for microflow LC-MS
August 2017

The following is a compendium of microflow LC-MS-based peer reviewed journal articles using Waters® ionKey/MS™ System. Each article is authored by leading researchers and/or Waters’ scientists. They cover many analytes of interest including small and large molecule therapeutics, biomarkers, supplements, and food.

High Throughput Microflow LC-MS: Sensitivity Gains on a Practical Timescale

The sensitivity gains observed when using microflow LC-MS do not always translate into an everyday workflow advantage in a high throughput laboratory. The longer run times that are typical on a microflow scale deliver improved sensitivity, but decreased sample throughput when compared to UPLC. The ability to deliver high sensitivity at run times under 3 minutes compelled the need to develop a microflow device that can function on a UPLC timescale (<3 min).

This white paper introduces a novel microfluidic device capable of higher pressures and flow rates which enable run times under 3 minutes while still delivering improved sensitivity over traditional UPLC systems.

Analysis of Aldosterone Using a Highly Analytically Sensitive Tandem Quadrupole MS for Clinical Research

Presented at ASMS 2016, this poster demonstrates that the performance of UPLC coupled to a newly-developed tandem quadrupole MS has been shown to provide quantification down to 2 pg/mL aldosterone using only 200 µL of a serum sample.